ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells
Salk Institute for Biological Studies · University of California San Diego · +1 more institution
Abstract
The chromatin structure of DNA determines genome compaction and activity in the nucleus. On the basis of in vitro structures and electron microscopy (EM) studies, the hierarchical model is that 11-nanometer DNA-nucleosome polymers fold into 30- and subsequently into 120- and 300- to 700-nanometer fibers and mitotic chromosomes. To visualize chromatin in situ, we identified a fluorescent dye that stains DNA with an osmiophilic polymer and selectively enhances its contrast in EM. Using ChromEMT (ChromEM tomography), we reveal the ultrastructure and three-dimensional (3D) organization of individual chromatin polymers, megabase domains, and mitotic chromosomes. We show that chromatin is a disordered 5- to…
Citation impact
- FWCI
- 45.30
- Percentile
- 100%
- References
- 97
Authors
6Topics & keywords
- Interphase
- Mitosis
- Chromatin
- Cell biology
- Biology
- Chemistry
- Computational biology
- Genetics
Funding
- HHHoward Hughes Medical InstituteAward: award297101
- WMW. M. Keck FoundationAward: award297105
- LMLeona M. and Harry B. Helmsley Charitable TrustAward: award297106
- LALouis and Harold Price FoundationAward: award297107
- NONIH Office of the DirectorAwards: 5U01EB021247, award297102
- NCNational Cancer InstituteAwards: P30CA014195, award297103
- NINational Institute of General Medical SciencesAwards: award297104, GM103412