Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function
Max Delbrück Center · Charité - Universitätsmedizin Berlin · +1 more institution
Abstract
Cutting out circular RNAs Circular RNAs are widespread, but their functions have been controversial. Piwecka et al. used CRISPR-Cas9 technology to remove the locus encoding the circular RNA Cdr1as from the mouse genome. Single-cell electrophysiological measurements in excitatory neurons revealed an increase in spontaneous vesicle release from the knockout mice and depression in the synaptic response with two consecutive stimuli, indicating that Cdr1as deficiency leads to dysfunction of excitatory synaptic transmission. Small RNA sequencing of several major regions of the brain showed that expression of two microRNAs, miR-7 and miR-671, that bind to Cdr1as decreased and increased, respectively. These results,…
Citation impact
- FWCI
- 54.83
- Percentile
- 100%
- References
- 65
Authors
20Topics & keywords
- microRNA
- Biology
- Phenotype
- Loss function
- Genetics
- Locus (genetics)
- Gene
- Neuroscience