Comprehensive single-cell transcriptional profiling of a multicellular organism

At the L2 larval stage, which provided >50-fold "shotgun" cellular coverage of its somatic cell composition. From these data, we defined consensus expression profiles for 27 cell types and recovered rare neuronal cell types corresponding to as few as one or two cells in the L2 worm. We integrated these profiles with whole-animal chromatin immunoprecipitation sequencing data to deconvolve the cell type-specific effects of transcription factors. The data generated by sci-RNA-seq constitute a powerful resource for nematode biology and foreshadow similar atlases for other organisms.

• HH
  Howard Hughes Medical Institute

  Award: award336988
• WM
  W. M. Keck Foundation

  Awards: award337002, award300298
• PG
  Paul G. Allen Family Foundation

  Award: award336997
• NI
  National Institutes of Health

  Awards: R01HG006283, award300293, DP1HG007811, U41HG007355, award300285, award300296, award336992, award300288, R01GM072675, DP2 HD088158
• NH
  National Heart, Lung, and Blood Institute

  Awards: T32HL007828, award300306