Gavage of Fecal Samples From Patients With Colorectal Cancer Promotes Intestinal Carcinogenesis in Germ-Free and Conventional Mice
Chinese University of Hong Kong · Chinese University of Hong Kong, Shenzhen · +2 more institutions
Abstract
Background & AimsAltered gut microbiota is implicated in development of colorectal cancer (CRC). Some intestinal bacteria have been reported to potentiate intestinal carcinogenesis by producing genotoxins, altering the immune response and intestinal microenvironment, and activating oncogenic signaling pathways. We investigated whether stool from patients with CRC could directly induce colorectal carcinogenesis in mice.MethodsWe obtained stored stool samples from participants in a metagenome study performed in Hong Kong. Conventional (male C57BL/6) mice were given azoxymethane to induce colon neoplasia after receiving a course of antibiotics in drinking water. Mice were gavaged twice weekly with stool from 5…
Citation impact
- FWCI
- 19.06
- Percentile
- 100%
- References
- 39
Authors
15- SHSunny H. Wong
Chinese University of Hong Kong, Chinese University of Hong Kong, Shenzhen
- LZLiuyang Zhao
Chinese University of Hong Kong, Chinese University of Hong Kong, Shenzhen
- XZXiang Zhang
Chinese University of Hong Kong, Chinese University of Hong Kong, Shenzhen
- GNGeicho Nakatsu
Chinese University of Hong Kong, Chinese University of Hong Kong, Shenzhen
- JHJuqiang Han
Chinese University of Hong Kong, Chinese University of Hong Kong, Shenzhen, The Military General Hospital of Beijing PLA
Topics & keywords
- Colorectal cancer
- Azoxymethane
- Feces
- Carcinogenesis
- Gastroenterology
- Dysplasia
- Calprotectin
- Biology
- Clean water and sanitation