Circular Noncoding RNA HIPK3 Mediates Retinal Vascular Dysfunction in Diabetes Mellitus
Tongji University · Eye & ENT Hospital of Fudan University · +6 more institutions
Abstract
The vascular complications of diabetes mellitus are the major causes of morbidity and mortality among people with diabetes. Circular RNAs are a class of endogenous noncoding RNAs that regulate gene expression in eukaryotes. In this study, we investigated the role of circular RNA in retinal vascular dysfunction induced by diabetes mellitus.
Quantitative polymerase chain reactions, Sanger sequencing, and Northern blots were conducted to detect circular HIPK3 (circHIPK3) expression pattern on diabetes mellitus-related stresses. MTT (3-[4,5-dimethythiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assays, EdU (5-ethynyl-2'-deoxyuridine) incorporation assays, Transwell migration assays, and Matrigel assays were conducted to detect the role of circHIPK3 in retinal endothelial cell function in vitro. Retinal trypsin digestion, vascular permeability assays, and ELISA assays were conducted to detect the role of circHIPK3 in retinal vascular dysfunction in vivo. Bioinformatics analysis, luciferase activity assays, RNA pull-down assays, and in vitro studies were conducted to reveal the mechanism of circHIPK3-mediated retinal vascular dysfunction.
Citation impact
- FWCI
- 24.56
- Percentile
- 100%
- References
- 36
Authors
14- KYK. Y. ShanCorresponding
Tongji University, Eye & ENT Hospital of Fudan University, Shanghai East Hospital, Shanghai Tenth People's Hospital, Second Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Children's Hospital of Fudan University, Nanjing Medical University
- CLChang Liu
Tongji University, Eye & ENT Hospital of Fudan University, Shanghai East Hospital, Shanghai Tenth People's Hospital, Second Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Children's Hospital of Fudan University, Nanjing Medical University
- BLBai-Hui Liu
Tongji University, Eye & ENT Hospital of Fudan University, Shanghai East Hospital, Shanghai Tenth People's Hospital, Second Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Children's Hospital of Fudan University, Nanjing Medical University
- XCXue Chen
Tongji University, Eye & ENT Hospital of Fudan University, Shanghai East Hospital, Shanghai Tenth People's Hospital, Second Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Children's Hospital of Fudan University, Nanjing Medical University
- RDRui Dong
Tongji University, Eye & ENT Hospital of Fudan University, Shanghai East Hospital, Shanghai Tenth People's Hospital, Second Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Children's Hospital of Fudan University, Nanjing Medical University
Topics & keywords
- Retinal
- Diabetes mellitus
- Gene silencing
- Retina
- Vascular permeability
- Medicine
- Endothelial stem cell
- Vascular endothelial growth factor
- Good health and well-being