The New Xpert MTB/RIF Ultra: Improving Detection of Mycobacterium tuberculosis and Resistance to Rifampin in an Assay Suitable for Point-of-Care Testing
Rutgers, The State University of New Jersey · Rutgers New Jersey Medical School · +6 more institutions
Abstract
ABSTRACT The Xpert MTB/RIF assay (Xpert) is a rapid test for tuberculosis (TB) and rifampin resistance (RIF-R) suitable for point-of-care testing. However, it has decreased sensitivity in smear-negative sputum, and false identification of RIF-R occasionally occurs. We developed the Xpert MTB/RIF Ultra assay (Ultra) to improve performance. Ultra and Xpert limits of detection (LOD), dynamic ranges, and RIF-R rpoB mutation detection were tested on Mycobacterium tuberculosis DNA or sputum samples spiked with known numbers of M. tuberculosis H37Rv or Mycobacterium bovis BCG CFU. Frozen and prospectively collected clinical samples from patients suspected of having TB, with and without culture-confirmed TB, were also…
Citation impact
- FWCI
- 35.13
- Percentile
- 100%
- References
- 23
Authors
25- SCSoumitesh ChakravortyCorresponding
Rutgers, The State University of New Jersey, Rutgers New Jersey Medical School
- AMAnn Marie Simmons
- MRMazhgan Rowneki
Rutgers, The State University of New Jersey, Rutgers New Jersey Medical School
- HPHeta Parmar
Rutgers, The State University of New Jersey, Rutgers New Jersey Medical School
- YCYuan Cao
Rutgers, The State University of New Jersey, Rutgers New Jersey Medical School
Topics & keywords
- Mycobacterium tuberculosis
- Point-of-care testing
- Point of care
- GeneXpert MTB/RIF
- Tuberculosis
- Medicine
- Virology
- Microbiology
- Good health and well-being