Guidelines for the use of flow cytometry and cell sorting in immunological studies  *

Cossarizza, Andrea; Chang, Hyun‐Dong; Radbruch, Andreas; Akdiş, Mübeccel; Andrä, Immanuel; Annunziato, Francesco; Bächer, Petra; Barnaba, Vincenzo; Battistini, Luca; Bauer, Wolfgang; Baumgart, Sabine; Becher, Burkhard; Beisker, Wolfgang; Berek, Claudia; Blanco, Alfonso; Borsellino, Giovanna; Boulais, Philip E.; Brinkman, Ryan R.; Büscher, Martin; Busch, Dirk H.; Bushnell, Timothy; Cao, Xuetao; Cavani, Andrea; Chattopadhyay, Pratip K.; Cheng, Qingyu; Chow, Sue; Clerici, Mario; Cooke, Anne; Cosma, Antonio; Cosmi, Lorenzo; Cumano, Ana; Dang, Van Duc; Davies, Derek; Biasi, Sara De; Zotto, Genny Del; Bella, Silvia Della; Dellabona, Paolo; Deniz, Günnur; Dessing, Mark C.; Diefenbach, Andreas; Santo, James P. Di; Dieli, Francesco; Dolf, Andreas; Donnenberg, Vera S.; Dörner, Thomas; Ehrhardt, Götz R. A.; Endl, Elmar; Engel, Pablo; Engelhardt, Britta; Esser, Charlotte; Everts, Bart; Dreher, Anita; Falk, Christine S.; Fehniger, Todd A.; Filby, Andrew; Fillatreau, Simon; Follo, Marie; Förster, Irmgard; Foster, John R.; Foulds, Gemma A.; Frenette, Paul S.; Galbraith, David W.; Garbi, Natalio; García‐Godoy, Maria Dolores; Geginat, Jens; Ghoreschi, Kamran; Gibellini, Lara; Goettlinger, Christoph; Goodyear, Carl S.; Gori, Andrea; Grogan, Jane L.; Gross, Mor; Grützkau, Andreas; Grummitt, Daryl; Hahn, Jonas; Hammer, Quirin; Hauser, Anja E.; Haviland, David L.; Hedley, David W.; Herrera, Guadalupe; Herrmann, Martin; Hiepe, Falk; Holland, Tristan; Hombrink, Pleun; Houston, Jessica P.; Hoyer, Bimba F.; Huang, Bo; Hunter, Christopher A.; Iannone, Anna; Jäck, Hans‐Martin; Jávega, Beatriz; Jonjić, Stipan; Juelke, Kerstin; Jung, Steffen; Kaiser, Toralf; Kalina, Tomáš; Keller, Baerbel; Khan, Srijit; Kienhöfer, Deborah; Kroneis, Thomas; Kunkel, Désirée; Kurts, Christian; Kvistborg, Pia; Lannigan, Joanne; Lantz, Olivier; Larbi, Anis; LeibundGut‐Landmann, Salomé; Leipold, Michael D.; Levings, Megan K.; Litwin, Virginia; Liu, Yanling; Lohoff, Michael; Lombardi, Giovanna; Lopez, Lilly; Lovett‐Racke, Amy E.; Lubberts, Erik; Ludewig, Burkhard; Lugli, Enrico; Maecker, Holden T.; Martrus, Glòria; Matarese, Giuseppe; Maueröder, Christian; McGrath, Mairi; McInnes, Iain B.; Mei, Henrik E.; Melchers, Fritz; Melzer, Susanne; Mielenz, Dirk; Mills, Kingston H. G.; Mirrer, David; Mjösberg, Jenny; Moore, Jonni S.; Moran, Barry; Moretta, Alessandro; Moretta, Lorenzo; Mosmann, Tim R.; Müller, Susann; Müller, Werner; Münz, Christian; Multhoff, Gabriele; Muñoz, Luis E.; Murphy, Kenneth M.; Nakayama, Toshinori; Nasi, Milena; Neudörfl, Christine; Nolan, John P.; Nourshargh, Sussan; O’Connor, José‐Enrique; Ouyang, Wenjun; Oxenius, Annette; Palankar, Raghavendra; Panse, Isabel; Peterson, Pärt; Peth, Christian; Pétriz, Jordi; Philips, Daisy; Pickl, Winfried F.; Piconese, Silvia; Pinti, Marcello; Pockley, A. Graham; Podolska, Malgorzata J.; Pucillo, Carlo; Quataert, Sally A.; Radstake, Timothy R. D. J.; Rajwa, Bartek; Rebhahn, Jonathan A.; Recktenwald, Diether; Remmerswaal, Ester B. M.; Rezvani, Katy; Rico, Laura G.; Robinson, J. Paul; Romagnani, Chiara; Rubartelli, Anna; Rückert, Beate; Ruland, Jürgen; Sakaguchi, Shimon; Sala‐de‐Oyanguren, Francisco; Samstag, Yvonne; Sanderson, Sharon; Sawitzki, Birgit; Scheffold, Alexander; Schiemann, Matthias; Schildberg, Frank A.; Schimisky, Esther; Schmid, Stephan; Schmitt, Steffen; Schober, Kilian; Schüler, Thomas; Schulz, Axel; Schumacher, Ton N.; Scottà, Cristiano; Shankey, T. Vincent; Shemer, Anat; Simon, Anna Katharina; Špidlen, Josef; Stall, Alan M.; Stark, Regina; Stehle, Christina; Stein, Merle; Steinmetz, Tobit; Stockinger, Hannes; Takahama, Yousuke; Tárnok, Attila; Tian, Zhi‐Gang; Toldi, Gergely; Tornack, Julia; Traggiai, Elisabetta; Trotter, Joe; Ulrich, Henning; Braber, Marlous van der; Lier, René A. W. van; Veldhoen, Marc; Vento‐Asturias, Salvador; Vieira, Paulo; Voehringer, David; Volk, Hans‐Dieter; Volkmann, Konrad von; Waisman, Ari; Walker, Rachael; Ward, Michael D.; Warnatz, Klaus; Warth, Sarah; Watson, James; Watzl, Carsten; Wegener, Leonie; Wiedemann, Annika; Wienands, Jürgen; Willimsky, Gerald; Wing, James B.; Wurst, Peter; Yu, Liping; Yue, Alice; Zhang, Qianjun; Zhao, Yi; Ziegler, Susanne; Zimmermann, Jakob

doi:10.1002/eji.201646632

articleEuropean Journal of ImmunologyOct 1, 2017BRONZE OA

Guidelines for the use of flow cytometry and cell sorting in immunological studies *

ACAndrea Cossarizza HCHyun‐Dong Chang ARAndreas Radbruch MAMübeccel Akdiş IAImmanuel Andrä

University of Modena and Reggio Emilia · Leibniz Association · +162 more institutions

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Abstract

Funding Information: Mairi Mc Grath and Regina Stark thank Francesco Siracusa and Patrick Maschmeyer for providing data and Klaas van Gisbergen for helpful discussions. Philip E. Boulais and Paul S. Frenette are grateful to Dr. Sandra Pinho for helpful comments and suggestions. They thank the National Institutes of Health for their support (R01 grants DK056638, HL116340, HL097819 to P.S.F). They also thank the New York State Department of Health (NYSTEM Program) for shared facility (C029154) and research support (N13G-262) and the Leukemia and Lymphoma Society’s Translational Research Program. Funding Information: Acknowledgements: Enrico Lugli and Pratip K. Chattopadhyay were supported by grants from the…

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Topics & keywords

Topics

Keywords

Biology
Flow cytometry
Sorting
Cell sorting
Mass cytometry
Computational biology
Immunology
Genetics

UN Sustainable Development Goals

Good health and well-being

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