Multiscale 3D Genome Rewiring during Mouse Neural Development
Centre National de la Recherche Scientifique · Institut de Génétique Humaine · +6 more institutions
Abstract
Chromosome conformation capture technologies have revealed important insights into genome folding. Yet, how spatial genome architecture is related to gene expression and cell fate remains unclear. We comprehensively mapped 3D chromatin organization during mouse neural differentiation in vitro and in vivo, generating the highest-resolution Hi-C maps available to date. We found that transcription is correlated with chromatin insulation and long-range interactions, but dCas9-mediated activation is insufficient for creating TAD boundaries de novo. Additionally, we discovered long-range contacts between gene bodies of exon-rich, active genes in all cell types. During neural differentiation, contacts between active…
Citation impact
- FWCI
- 61.22
- Percentile
- 100%
- References
- 53
Authors
11- BBBoyan BonevCorresponding
Centre National de la Recherche Scientifique, Institut de Génétique Humaine
- NMNetta Mendelson Cohen
Weizmann Institute of Science
- QSQuentin Szabo
Centre National de la Recherche Scientifique, Université de Montpellier, Institut de Génétique Humaine
- LFLauriane Fritsch
Centre National de la Recherche Scientifique, Université de Montpellier, Institut de Génétique Humaine
- GLGiorgio L. Papadopoulos
Centre National de la Recherche Scientifique, Université de Montpellier, Institut de Génétique Humaine
Topics & keywords
- Biology
- Chromatin
- Enhancer
- Chromosome conformation capture
- Genome
- Gene
- Transcription factor
- Genetics