Coding and noncoding landscape of extracellular RNA released by human glioma stem cells

Tumor-released RNA may mediate intercellular communication and serve as biomarkers. Here we develop a protocol enabling quantitative, minimally biased analysis of extracellular RNAs (exRNAs) associated with microvesicles, exosomes (collectively called EVs), and ribonucleoproteins (RNPs). The exRNA complexes isolated from patient-derived glioma stem-like cultures exhibit distinct compositions, with microvesicles most closely reflecting cellular transcriptome. exRNA is enriched in small ncRNAs, such as miRNAs in exosomes, and precisely processed tRNA and Y RNA fragments in EVs and exRNPs. EV-enclosed mRNAs are mostly fragmented, and UTRs enriched; nevertheless, some full-length mRNAs are present. Overall, there…

• NI
  National Institutes of Health

  Awards: CA069246, NS098051, U19 CA179563, CA179563
• NO
  NIH Office of the Director

  Awards: R21 NS098051, U19 CA179563, CA179563
• OO
  Office of Strategic Coordination

  Award: U19 CA179563
• CF
  Common Fund