Super-Resolution Structured Illumination Microscopy
Leibniz Institute of Photonic Technology · Friedrich Schiller University Jena · +2 more institutions
Abstract
Super-resolved structured illumination microscopy (SR-SIM) is among the most rapidly growing fluorescence microscopy techniques that can surpass the optical diffraction limit. The strength of SR-SIM is that it can be readily applied to samples prepared for conventional fluorescence microscopy, requiring no sophisticated sample preparation protocols. As an extension of wide-field fluorescence microscopy, it is inherently capable of multicolor imaging and optical sectioning and, with sufficiently fast implementations, permits live cell imaging. Image reconstruction, however, currently relies on sophisticated computational procedures, susceptible to reconstruction artifacts, requiring trained users to recognize…
Citation impact
- FWCI
- 32.27
- Percentile
- 100%
- References
- 100
Authors
2Topics & keywords
- Microscopy
- Fluorescence microscope
- Resolution (logic)
- Nanotechnology
- Optical sectioning
- Optical microscope
- Computer science
- Superresolution