Structural insight into catalytic mechanism of PET hydrolase
Chinese Academy of Sciences · Tianjin Institute of Industrial Biotechnology · +2 more institutions
Abstract
PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.
Citation impact
- FWCI
- 7.70
- Percentile
- 100%
- References
- 23
Authors
10- XHXu HanCorresponding
Chinese Academy of Sciences, Tianjin Institute of Industrial Biotechnology
- WLWeidong Liu
Chinese Academy of Sciences, Tianjin Institute of Industrial Biotechnology
- JHJian‐Wen Huang
- JMJiantao Ma
Jiangnan University, Chinese Academy of Sciences, Tianjin Institute of Industrial Biotechnology
- YZYingying Zheng
Chinese Academy of Sciences, Tianjin Institute of Industrial Biotechnology
Topics & keywords
- Hydrolase
- Polyethylene terephthalate
- Substrate (aquarium)
- Bioconversion
- Mutagenesis
- Catalysis
- Substrate specificity
- Chemistry