Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

Boeynaems, Steven; Holehouse, Alex S.; Weinhardt, Venera; Kovács, Dénes; Lindt, Joris Van; Larabell, Carolyn A.; Bosch, Ludo Van Den; Das, Rhiju; Tompa, Péter; Pappu, Rohit V.; Gitler, Aaron D.

doi:10.1073/pnas.1821038116

articleProceedings of the National Academy of SciencesMar 29, 2019HYBRID OA

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

SBSteven Boeynaems ASAlex S. Holehouse VWVenera Weinhardt DKDénes Kovács JVJoris Van Lindt

Stanford University · Washington University in St. Louis · +9 more institutions

PubMed

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Abstract

Phase separation of multivalent protein and RNA molecules underlies the biogenesis of biomolecular condensates such as membraneless organelles. In vivo, these condensates encompass hundreds of distinct types of molecules that typically organize into multilayered structures supporting the differential partitioning of molecules into distinct regions with distinct material properties. The interplay between driven (active) versus spontaneous (passive) processes that are required for enabling the formation of condensates with coexisting layers of distinct material properties remains unclear. Here, we deploy systematic experiments and simulations based on coarse-grained models to show that the collective…

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Topics

Keywords

Biogenesis
RNA
Molecule
Biological system
Chemical physics
Phase (matter)
Chemistry
Biophysics

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