articleCurrent Protocols in BioinformaticsDec 1, 2014Closed access

Clustal Omega

FSFabian SieversDGDesmond G. Higgins

University College Dublin

PubMed
Indexed incrossrefpubmed

Abstract

Clustal Omega is a package for making multiple sequence alignments of amino acid or nucleotide sequences, quickly and accurately. It is a complete upgrade and rewrite of earlier Clustal programs. This unit describes how to run Clustal Omega interactively from a command line, although it can also be run online from several sites. The unit describes a basic protocol for taking a set of unaligned sequences and producing a full alignment. There are also protocols for using an external HMM or iteration to help improve an alignment.

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Topics & keywords

Topics
Keywords
  • Omega
  • Computer science
  • Set (abstract data type)
  • Multiple sequence alignment
  • Upgrade
  • Line (geometry)
  • Protocol (science)
  • Sequence alignment
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