Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart

The heart is the central organ of the circulatory system, and its proper development is vital for maintaining human life. Here, we used single-cell RNA sequencing to profile the gene expression landscapes of ∼4,000 cardiac cells from human embryos and identified four major types of cells: cardiomyocytes (CMs), cardiac fibroblasts, endothelial cells (ECs), and valvar interstitial cells (VICs). Atrial and ventricular CMs acquired distinct features early in heart development. Furthermore, both CMs and fibroblasts show stepwise changes in gene expression. As development proceeds, VICs may be involved in the remodeling phase, and ECs display location-specific characteristics. Finally, we compared gene expression…

• NN
  National Natural Science Foundation of China

  Awards: 31230047, 31625018, 31571544, 81521002, 31522034
• PU
  Peking University
• BM
  Beijing Municipal Science and Technology Commission

  Award: D151100002415000