Excessive Cell Growth Causes Cytoplasm Dilution And Contributes to Senescence
Howard Hughes Medical Institute · Massachusetts Institute of Technology · +8 more institutions
Abstract
Cell size varies greatly between cell types, yet within a specific cell type and growth condition, cell size is narrowly distributed. Why maintenance of a cell-type specific cell size is important remains poorly understood. Here we show that growing budding yeast and primary mammalian cells beyond a certain size impairs gene induction, cell-cycle progression, and cell signaling. These defects are due to the inability of large cells to scale nucleic acid and protein biosynthesis in accordance with cell volume increase, which effectively leads to cytoplasm dilution. We further show that loss of scaling beyond a certain critical size is due to DNA becoming limiting. Based on the observation that senescent cells…
Citation impact
- FWCI
- 21.19
- Percentile
- 100%
- References
- 54
Authors
16- GEGabriel E. Neurohr
Howard Hughes Medical Institute, Massachusetts Institute of Technology
- RTRachel Terry
Howard Hughes Medical Institute, Harvard University, Center for Systems Biology, Massachusetts Institute of Technology
- JLJette Lengefeld
Howard Hughes Medical Institute, Massachusetts Institute of Technology
- MBMegan Bonney
Foundation for Biomedical Research, Howard Hughes Medical Institute, Novartis (United States), Massachusetts Institute of Technology
- GBGregory Brittingham
NYU Langone Health
Topics & keywords
- Biology
- Cytoplasm
- Cell
- Cell biology
- Cell cycle
- Cell growth
- Cell type
- Senescence
- Life in Land