The human gut bacterial genotoxin colibactin alkylates DNA

Wilson, Matthew R.; Jiang, Yindi; Villalta, Peter W.; Stornetta, Alessia; Boudreau, Paul D.; Carrà, Andrea; Brennan, Caitlin A.; Chun, Eunyoung; Ngo, Lizzie; Samson, Leona D.; Engelward, Bevin P.; Garrett, Wendy S.; Balbo, Silvia; Balskus, Emily P.

doi:10.1126/science.aar7785

articleScienceFeb 14, 2019BRONZE OA

The human gut bacterial genotoxin colibactin alkylates DNA

MRMatthew R. Wilson YJYindi Jiang PWPeter W. Villalta ASAlessia Stornetta PDPaul D. Boudreau

Harvard University · University of Minnesota Medical Center · +3 more institutions

PubMed

Indexed incrossrefpubmed

Abstract

Bacterial warhead targets DNA The bacterial toxin colibactin causes double-stranded DNA breaks and is associated with the occurrence of bacterially induced colorectal cancer in humans. However, isolation of colibactin is difficult, and its mode of action is poorly understood. Wilson et al. studied Escherichia coli that contain the biosynthetic gene island called pks , which is associated with colibactin production (see the Perspective by Bleich and Arthur). They identified the DNA adducts that resulted from incubating pks + E. coli in human cells. To overcome the lack of colibactin for direct analysis, mimics of the pks product were synthesized. From the resulting synthetic adenine-colibactin adducts, it…

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Topics & keywords

Topics

Keywords

Biology
DNA
Escherichia coli
DNA damage
Genetics
Metabolite
DNA adduct
Carcinogenesis

UN Sustainable Development Goals

Life below water

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