Nucleic Acid-Functionalized Metal–Organic Framework-Based Homogeneous Electrochemical Biosensor for Simultaneous Detection of Multiple Tumor Biomarkers

With target miRNAs impel the generation of RNA-DNA complexes, which separated from MOFs and allowed the electroactive dyes to be released. In comparison with the case when target miRNAs are absent, two stronger signals are recorded, and dependent on target miRNA concentrations. Thus, simultaneous detection of let-7a and minRNA-21 is achieved, with detection limits down to 3.6 and 8.2 fM, respectively, comparable or lower than those of reported strategies that concentrated on single miRNA detection. Moreover, the proposed biosensor has also been successfully applied for simultaneous detection of target miRNAs spiked in serum samples. Therefore, the proposed strategy was expected to provide more information for…

• NN
  National Natural Science Foundation of China

  Awards: 21605093, 21575074, 21775082
• NS
  Natural Science Foundation of Shandong Province

  Award: ZR2018ZC0127
• GO
  Government of Shandong Province

  Award: ts201511052