Increased glymphatic influx is correlated with high EEG delta power and low heart rate in mice under anesthesia

The glymphatic system is responsible for brain-wide delivery of nutrients and clearance of waste via influx of cerebrospinal fluid (CSF) alongside perivascular spaces and through the brain. Glymphatic system activity increases during sleep or ketamine/xylazine (K/X) anesthesia, yet the mechanism(s) facilitating CSF influx are poorly understood. Here, we correlated influx of a CSF tracer into the brain with electroencephalogram (EEG) power, heart rate, blood pressure, and respiratory rate in wild-type mice under six different anesthesia regimens. We found that glymphatic CSF tracer influx was highest under K/X followed by isoflurane (ISO) supplemented with dexmedetomidine and pentobarbital. Mice anesthetized…

• DM
  Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
• NN
  Novo Nordisk UK Research Foundation
• L
  Lundbeckfonden
• SJ
  Sigrid Juséliuksen Säätiö
• NI
  National Institute on Aging

  Award: RF1AG057575
• NI
  National Institute of Neurological Disorders and Stroke

  Award: R01NS100366