Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice
State Key Laboratory of Plant Genomics · Institute of Genetics and Developmental Biology · +6 more institutions
Abstract
Spotting off-targets from gene editing Unintended genomic modifications limit the potential therapeutic use of gene-editing tools. Available methods to find off-targets generally do not work in vivo or detect single-nucleotide changes. Three papers in this issue report new methods for monitoring gene-editing tools in vivo (see the Perspective by Kempton and Qi). Wienert et al. followed the recruitment of a DNA repair protein to DNA breaks induced by CRISPR-Cas9, enabling unbiased detection of off-target editing in cellular and animal models. Zuo et al. identified off-targets without the interference of natural genetic heterogeneity by injecting base editors into one blastomere of a two-cell mouse embryo and…
Citation impact
- FWCI
- 35.97
- Percentile
- 100%
- References
- 34
Authors
11- SJShuai JinCorresponding
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Institute of Genetics, University of Chinese Academy of Sciences
- YZYuan ZongCorresponding
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Institute of Genetics, University of Chinese Academy of Sciences
- QGQiang GaoCorresponding
State Key Laboratory of Plant Genomics
- ZZZixu Zhu
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Institute of Genetics, University of Chinese Academy of Sciences
- YWYanpeng Wang
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Institute of Genetics
Topics & keywords
- Genome
- Biology
- Computational biology
- Genetics
- Gene