reviewAnnual Review of Plant BiologyMar 5, 2019BRONZE OA

CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

Chinese Academy of Sciences · Institute of Genetics and Developmental Biology · +1 more institution

PubMed
Indexed incrossrefpubmed

Abstract

Enhanced agricultural production through innovative breeding technology is urgently needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas genome editing enable efficient targeted modification in most crops, thus promising to accelerate crop improvement. Here, we review advances in CRISPR/Cas9 and its variants and examine their applications in plant genome editing and related manipulations. We highlight base-editing tools that enable targeted nucleotide substitutions and describe the various delivery systems, particularly DNA-free methods, that have linked genome editing with crop breeding. We summarize the applications of genome editing for trait improvement, development of…

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