articleCellMar 1, 2019HYBRID OA

Characterizing Mutational Signatures in Human Cancer Cell Lines Reveals Episodic APOBEC Mutagenesis

Wellcome Sanger Institute · University of California San Diego · +8 more institutions

PubMed
Indexed incrossrefdatacitepubmed

Abstract

Multiple signatures of somatic mutations have been identified in cancer genomes. Exome sequences of 1,001 human cancer cell lines and 577 xenografts revealed most common mutational signatures, indicating past activity of the underlying processes, usually in appropriate cancer types. To investigate ongoing patterns of mutational-signature generation, cell lines were cultured for extended periods and subsequently DNA sequenced. Signatures of discontinued exposures, including tobacco smoke and ultraviolet light, were not generated in vitro. Signatures of normal and defective DNA repair and replication continued to be generated at roughly stable mutation rates. Signatures of APOBEC cytidine deaminase DNA-editing…

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444
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18.47
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100%
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84
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Authors

35

Topics & keywords

Keywords
  • Biology
  • APOBEC
  • Genetics
  • Mutagenesis
  • Cytidine deaminase
  • Retrotransposon
  • Somatic hypermutation
  • Mutation
UN Sustainable Development Goals
  • Good health and well-being
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