CUT&Tag for efficient epigenomic profiling of small samples and single cells

Many chromatin features play critical roles in regulating gene expression. A complete understanding of gene regulation will require the mapping of specific chromatin features in small samples of cells at high resolution. Here we describe Cleavage Under Targets and Tagmentation (CUT&Tag), an enzyme-tethering strategy that provides efficient high-resolution sequencing libraries for profiling diverse chromatin components. In CUT&Tag, a chromatin protein is bound in situ by a specific antibody, which then tethers a protein A-Tn5 transposase fusion protein. Activation of the transposase efficiently generates fragment libraries with high resolution and exceptionally low background. All steps from live cells to…

• HH
  Howard Hughes Medical Institute

  Award: TCPA A093
• FH
  Fred Hutchinson Cancer Research Center
• NI
  National Institutes of Health

  Award: 4DN TCPA A093
• OO
  Office of Extramural Research, National Institutes of Health

  Award: 4DN TCPA A093