Enhanced rice salinity tolerance via CRISPR/Cas9-targeted mutagenesis of the OsRR22 gene
Huazhong Agricultural University · Shanghai Agrobiological Gene Center
Abstract
Salinity is one of the most important abiotic stress affecting the world rice production. The cultivation of salinity-tolerant cultivars is the most cost-effective and environmentally friendly approach for salinity control. In recent years, CRISPR/Cas9 systems have been widely used for target-site genome editing; however, their application for the improvement of elite rice cultivars has rarely been reported. Here, we report the improvement of the rice salinity tolerance by engineering a Cas9-OsRR22-gRNA expressing vector, targeting the OsRR22 gene in rice. Nine mutant plants were identified from 14 T0 transgenic plants. Sequencing showed that these plants had six mutation types at the target site, all of which…
Citation impact
- FWCI
- 17.69
- Percentile
- 100%
- References
- 36
Authors
14- AZAnning ZhangCorresponding
Huazhong Agricultural University, Shanghai Agrobiological Gene Center
- YLYi Liu
Huazhong Agricultural University, Shanghai Agrobiological Gene Center
- FWFeiming Wang
Shanghai Agrobiological Gene Center
- TLTianfei Li
Shanghai Agrobiological Gene Center
- ZCZhihao Chen
Shanghai Agrobiological Gene Center
Topics & keywords
- CRISPR
- Biology
- Salinity
- Mutant
- Cas9
- Genetically modified rice
- Cultivar
- Mutagenesis