Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis for Highly Efficient PET Degradation
Kyungpook National University · Korea Advanced Institute of Science and Technology
Abstract
Widespread utilization of polyethylene terephthalate (PET) has caused a variety of environmental and health problems; thus, the enzymatic degradation of PET can be a promising solution. Although PETase from Ideonalla sakaiensis (IsPETase) has been reported to have the highest PET degradation activity under mild conditions of all PET-degrading enzymes reported to date, its low thermal stability limits its ability for efficient and practical enzymatic degradation of PET. Using the structural information on IsPETase, we developed a rational protein engineering strategy using several IsPETase variants that were screened for high thermal stability to improve PET degradation activity. In particular, the…
Citation impact
- FWCI
- 11.07
- Percentile
- 100%
- References
- 27
Authors
8Topics & keywords
- Degradation (telecommunications)
- Polyethylene terephthalate
- Thermal stability
- Rational design
- Protein engineering
- Enzyme
- Biodegradation
- Materials science