Whole genome experimental maps of DNA G-quadruplexes in multiple species

Genomic maps of DNA G-quadruplexes (G4s) can help elucidate the roles that these secondary structures play in various organisms. Herein, we employ an improved version of a G-quadruplex sequencing method (G4-seq) to generate whole genome G4 maps for 12 species that include widely studied model organisms and also pathogens of clinical relevance. We identify G4 structures that form under physiological K+ conditions and also G4s that are stabilized by the G4-targeting small molecule pyridostatin (PDS). We discuss the various structural features of the experimentally observed G-quadruplexes (OQs), highlighting differences in their prevalence and enrichment across species. Our study describes diversity in sequence…

• W
  Wellcome

  Award: 209441/z/17/z
• WT
  Wellcome Trust

  Award: 209441
• CR
  Cancer Research UK

  Awards: C9681/A18618, A17197, C14303, C14303/A17197, 209441/z/17/z
• UO
  University of Cambridge
• DF
  Directorate for Biological Sciences
• BA
  Biotechnology and Biological Sciences Research Council

  Award: BB/I015477/1
• ER
  European Research Council

  Award: 339778