Reconstitution and structure of a plant NLR resistosome conferring immunity

Coiled-coil NLR ZAR1, the pseudokinase RKS1, uridylated protein kinase PBL2, and 2'-deoxyadenosine 5'-triphosphate (dATP), demonstrating the oligomerization of the complex during immune activation. The cryo-electron microscopy structure reveals a wheel-like pentameric ZAR1 resistosome. Besides the nucleotide-binding domain, the coiled-coil domain of ZAR1 also contributes to resistosome pentamerization by forming an α-helical barrel that interacts with the leucine-rich repeat and winged-helix domains. Structural remodeling and fold switching during activation release the very N-terminal amphipathic α helix of ZAR1 to form a funnel-shaped structure that is required for the plasma membrane association, cell death…

• NN
  National Natural Science Foundation of China

  Awards: 31421001, 31700660
• CP
  China Postdoctoral Science Foundation

  Awards: 2017T100065, 2017M620746
• BM
  Beijing Municipal Science and Technology Commission

  Award: Z161100000116034