Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
Garvan Institute of Medical Research · The University of Melbourne · +6 more institutions
Abstract
Besides cardiomyocytes (CM), the heart contains numerous interstitial cell types which play key roles in heart repair, regeneration and disease, including fibroblast, vascular and immune cells. However, a comprehensive understanding of this interactive cell community is lacking. We performed single-cell RNA-sequencing of the total non-CM fraction and enriched (Pdgfra-GFP+) fibroblast lineage cells from murine hearts at days 3 and 7 post-sham or myocardial infarction (MI) surgery. Clustering of >30,000 single cells identified >30 populations representing nine cell lineages, including a previously undescribed fibroblast lineage trajectory present in both sham and MI hearts leading to a uniquely activated…
Citation impact
- FWCI
- 53.56
- Percentile
- 100%
- References
- 99
Authors
9- NFNona FarbehiCorresponding
Garvan Institute of Medical Research, The University of Melbourne, Victor Chang Cardiac Research Institute, UNSW Sydney, Stem Cells Australia
- RPRalph Patrick
The University of Melbourne, Victor Chang Cardiac Research Institute, UNSW Sydney, St Vincent's Clinic, Stem Cells Australia
- ADAude Dorison
The University of Melbourne, Victor Chang Cardiac Research Institute, Stem Cells Australia
- MXMunira Xaymardan
The University of Sydney, The University of Melbourne, Victor Chang Cardiac Research Institute, Westmead Hospital, Stem Cells Australia
- VJVaibhao Janbandhu
The University of Melbourne, Victor Chang Cardiac Research Institute, UNSW Sydney, St Vincent's Clinic, Stem Cells Australia
Topics & keywords
- Biology
- Cell biology
- Myofibroblast
- Fibroblast
- Cardiac fibrosis
- Cell type
- Stromal cell
- Wnt signaling pathway
- Good health and well-being
Funding
- AHAmerican Heart Association
- SVSt. Vincent's Clinic FoundationAward: 100711
- SCStem Cells AustraliaAward: SR110001002
- NHNational Heart Foundation of AustraliaAward: 100848
- FLFondation LeducqAwards: 15CVD03, 13CVD01
- UOUniversity of New South Wales
- NHNational Health and Medical Research CouncilAwards: 1074386, 1105271, 573707, 1118576