Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications
USA Mitchell Cancer Institute · University of South Alabama
Abstract
Exosomes have received significant attention for their role in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed for exosome isolation. Here we compared the efficacy of four kits from Invitrogen, 101Bio, Wako and iZON along with conventional ultracentrifugation-based method for exosome yield, purity and quality. Cell culture supernatant was used as an abundant source of exosomes, and exosome quantity, size-distribution, zeta-potential, marker-expression and RNA/protein quality were determined. The Invitrogen kit gave the highest yield but the preparation showed broader…
Citation impact
- FWCI
- 27.26
- Percentile
- 100%
- References
- 34
Authors
7- GKGirijesh Kumar PatelCorresponding
USA Mitchell Cancer Institute, University of South Alabama
- MAMohammad Aslam Khan
USA Mitchell Cancer Institute, University of South Alabama
- HZHaseeb Zubair
USA Mitchell Cancer Institute, University of South Alabama
- SKSanjeev K. Srivastava
USA Mitchell Cancer Institute, University of South Alabama
- MKMoh’d Khushman
USA Mitchell Cancer Institute, University of South Alabama
Topics & keywords
- Exosome
- Microvesicles
- Chemistry
- Chromatography
- RNA
- Polyethylene glycol
- Molecular biology
- Biochemistry