A high-quality apple genome assembly reveals the association of a retrotransposon and red fruit colour
Institute of Pomology · Nextomics Biosciences (China) · +2 more institutions
Abstract
Abstract A complete and accurate genome sequence provides a fundamental tool for functional genomics and DNA-informed breeding. Here, we assemble a high-quality genome (contig N50 of 6.99 Mb) of the apple anther-derived homozygous line HFTH1, including 22 telomere sequences, using a combination of PacBio single-molecule real-time (SMRT) sequencing, chromosome conformation capture (Hi-C) sequencing, and optical mapping. In comparison to the Golden Delicious reference genome, we identify 18,047 deletions, 12,101 insertions and 14 large inversions. We reveal that these extensive genomic variations are largely attributable to activity of transposable elements. Interestingly, we find that a long terminal repeat…
Citation impact
- FWCI
- 18.63
- Percentile
- 100%
- References
- 81
Authors
21Topics & keywords
- Retrotransposon
- Biology
- Genome
- Genetics
- Transposable element
- Contig
- Reference genome
- Sequence assembly
- Zero hunger