Native Chemical Ligation and Extended Methods: Mechanisms, Catalysis, Scope, and Limitations
Centre National de la Recherche Scientifique · Université de Lille · +4 more institutions
Abstract
The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester with an N-terminal cysteinyl peptide to produce a native peptide bond between the two fragments. This reaction has considerably extended the size of polypeptides and proteins that can be produced by total synthesis and has also numerous applications in bioconjugation, polymer synthesis, material science, and micro- and nanotechnology research. The aim of the present review is to provide a thorough mechanistic overview of NCL and extended methods. The most relevant properties of peptide thioesters, Cys peptides, and common solvents, reagents, additives, and catalysts used for these ligations are presented. Mechanisms,…
Citation impact
- FWCI
- 25.55
- Percentile
- 100%
- References
- 735
Authors
6- VAVangelis Agouridas
Centre National de la Recherche Scientifique, Université de Lille, Institut Pasteur de Lille, Center for Infection and Immunity of Lille
- OEOuafâa El Mahdi
Sidi Mohamed Ben Abdellah University
- VDVincent Diemer
Centre National de la Recherche Scientifique, Université de Lille, Institut Pasteur de Lille, Center for Infection and Immunity of Lille
- MCMarine Cargoët
Centre National de la Recherche Scientifique, Université de Lille, Institut Pasteur de Lille, Center for Infection and Immunity of Lille
- JMJean‐Christophe M. MonbaliuCorresponding
University of Liège
Topics & keywords
- Chemistry
- Thioester
- Native chemical ligation
- Bioconjugation
- Combinatorial chemistry
- Reactivity (psychology)
- Peptide
- Thiol