CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing

The CRISPR-Cas system is a powerful genome editing tool that functions in a diverse array of organisms and cell types. The technology was initially developed to induce targeted mutations in DNA, but CRISPR-Cas has now been adapted to target nucleic acids for a range of purposes. CHOPCHOP is a web tool for identifying CRISPR-Cas single guide RNA (sgRNA) targets. In this major update of CHOPCHOP, we expand our toolbox beyond knockouts. We introduce functionality for targeting RNA with Cas13, which includes support for alternative transcript isoforms and RNA accessibility predictions. We incorporate new DNA targeting modes, including CRISPR activation/repression, targeted enrichment of loci for long-read…

• ON
  Oxford Nanopore Technologies
• UI
  Universitetet i Bergen
• NF
  Norges Forskningsråd

  Award: 250049
• BF
  Bergens Forskningsstiftelse
• ND
  National Defense Science and Engineering Graduate