In vitro Cell Migration, Invasion, and Adhesion Assays: From Cell Imaging to Data Analysis
Universitat de Lleida · Instituto de Investigación Biomédica de Lleida · +4 more institutions
Abstract
Cell migration is a key procedure involved in many biological processes including embryological development, tissue formation, immune defense or inflammation and cancer progression. How physical, chemical and molecular aspects can affect cell motility is a challenge to understand migratory cells behavior. In vitro assays are excellent approaches to extrapolate to in vivo situations and study live cells behavior. Here we present four in vitro protocols that describe step-by-step cell migration, invasion and adhesion strategies and their corresponding image data quantification. These current protocols are based on two-dimensional wound healing assays (comparing traditional pipette tip-scratch assay vs. culture…
Citation impact
- FWCI
- 24.81
- Percentile
- 100%
- References
- 27
Authors
8- JPJordi Pijuan
Universitat de Lleida, Instituto de Investigación Biomédica de Lleida
- CBCarla Barceló
Universitat de Lleida, Instituto de Investigación Biomédica de Lleida
- DFDavid F. Moreno
Institut de Biologia Molecular de Barcelona
- ÓMÓscar Maiques
Universitat de Lleida, Instituto de Investigación Biomédica de Lleida
- PSPol Sisó
Universitat de Lleida, Instituto de Investigación Biomédica de Lleida
Topics & keywords
- Cell adhesion
- Cell migration
- Cell biology
- Cell
- In vitro
- Adhesion
- Live cell imaging
- Biology