Genome-wide cell-free DNA fragmentation in patients with cancer
Johns Hopkins University · Sidney Kimmel Comprehensive Cancer Center · +13 more institutions
Abstract
Cell-free DNA in the blood provides a non-invasive diagnostic avenue for patients with cancer1. However, characteristics of the origins and molecular features of cell-free DNA are poorly understood. Here we developed an approach to evaluate fragmentation patterns of cell-free DNA across the genome, and found that profiles of healthy individuals reflected nucleosomal patterns of white blood cells, whereas patients with cancer had altered fragmentation profiles. We used this method to analyse the fragmentation profiles of 236 patients with breast, colorectal, lung, ovarian, pancreatic, gastric or bile duct cancer and 245 healthy individuals. A machine learning model that incorporated genome-wide fragmentation…
Citation impact
- FWCI
- 45.67
- Percentile
- 100%
- References
- 42
Authors
36- SCStephen CristianoCorresponding
Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center
- ALAlessandro Leal
Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center
- JPJillian Phallen
Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center
- JFJacob Fiksel
Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University
- VAVilmos Adleff
Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center
Topics & keywords
- Fragmentation (computing)
- Genome
- DNA
- Genetics
- DNA fragmentation
- Biology
- Computational biology
- Gene
- Good health and well-being
Funding
- AAAmerican Association for Cancer ResearchAward: SU2C-AACR-DT1415
- DMDr. Miriam and Sheldon G. Adelson Medical Research FoundationAward: CA121113
- SFStrategiske ForskningsrådAward: 1309-00006B
- NNNovo NordiskAward: NNF14OC0012747
- DFDanmarks Frie Forskningsfond
- NNNovo Nordisk FondenAwards: NNF17OC0025052, NNF14OC0012747
- NINational Institutes of HealthAwards: CA006973, CA180950, CA121113
- SUStand Up To CancerAward: SU2C-AACR-DT1415