DNA barcode reference libraries for the monitoring of aquatic biota in Europe: Gap-analysis and recommendations for future work

Weigand, Hannah; Beermann, Arne J.; Čiampor, Fedor; Costa, Filipe O.; Csabai, Zoltán; Duarte, Sofia; Geiger, Matthias F.; Grabowski, Michał; Rimet, Frédéric; Rulik, Björn; Strand, Malin; Szucsich, Nikolaus U.; Weigand, Alexander; Willassen, Endre; Wyler, Sofia A.; Bouchez, Agnès; Borja, Ángel; Čiamporová‐Zaťovičová, Zuzana; Ferreira, Sónia; Dijkstra, Klaas‐Douwe B.; Eisendle, Ursula; Freyhof, Jörg; Gadawski, Piotr; Graf, Wolfram; Haegerbaeumer, Arne; Hoorn, Berry B. van der; Japoshvili, Bella; Keresztes, Lujza; Keskin, Emre; Leese, Florian; Macher, Jan‐Niklas; Mamos, Tomasz; Paz, Guy; Pešić, Vladimir; Pfannkuchen, Daniela Marić; Pfannkuchen, Martin; Price, Benjamin; Rinkevich, Buki; Teixeira, Marcos A. L.; Várbíró, Gábor; Ekrem, Torbjørn

doi:10.1016/j.scitotenv.2019.04.247

reviewThe Science of The Total EnvironmentApr 27, 2019HYBRID OA

DNA barcode reference libraries for the monitoring of aquatic biota in Europe: Gap-analysis and recommendations for future work

HWHannah Weigand AJArne J. Beermann FČFedor Čiampor FOFilipe O. Costa ZCZoltán Csabai

Musée National d'Histoire Naturelle · University of Duisburg-Essen · +31 more institutions

PubMed

Indexed incrossrefpubmed

Abstract

Effective identification of species using short DNA fragments (DNA barcoding and DNA metabarcoding) requires reliable sequence reference libraries of known taxa. Both taxonomically comprehensive coverage and content quality are important for sufficient accuracy. For aquatic ecosystems in Europe, reliable barcode reference libraries are particularly important if molecular identification tools are to be implemented in biomonitoring and reports in the context of the EU Water Framework Directive (WFD) and the Marine Strategy Framework Directive (MSFD). We analysed gaps in the two most important reference databases, Barcode of Life Data Systems (BOLD) and NCBI GenBank, with a focus on the taxa most frequently used…

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Topics & keywords

Topics

Keywords

Barcode
DNA barcoding
Marine Strategy Framework Directive
Context (archaeology)
Biota
Water Framework Directive
Biology
Identification (biology)

UN Sustainable Development Goals

Life below water

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