Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation*

Batth, Tanveer S.; Tollenaere, Maxim A. X.; Rüther, Patrick; González-Franquesa, Alba; Prabhakar, Bhargav S.; Bekker‐Jensen, Simon; Deshmukh, Atul S.; Olsen, Jesper V.

doi:10.1074/mcp.tir118.001270

articleMolecular & Cellular ProteomicsMar 4, 2019HYBRID OA

Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation*

TSTanveer S. Batth MAMaxim A. X. Tollenaere PRPatrick Rüther AGAlba González-Franquesa BSBhargav S. Prabhakar

Novo Nordisk Foundation · University of Copenhagen

PubMed

Indexed incrossrefdoajpubmed

Abstract

Universal proteomics sample preparation is challenging because of the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for nonspecific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings present a practical, sensitive and cost-effective proteomics sample preparation method. Universal proteomics sample preparation is challenging because of the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the…

Citation impact

476

total citations

FWCI: 14.10
Percentile: 100%
References: 23

Citations per year

Authors

8

Topics & keywords

Topics

Keywords

Proteomics
Sample preparation
Chemistry
Chromatography
Biochemistry

No related works found for this paper.