METTL3 facilitates tumor progression via an m6A-IGF2BP2-dependent mechanism in colorectal carcinoma

Colorectal carcinoma (CRC) is one of the most common malignant tumors, and its main cause of death is tumor metastasis. RNA N6-methyladenosine (m6A) is an emerging regulatory mechanism for gene expression and methyltransferase-like 3 (METTL3) participates in tumor progression in several cancer types. However, its role in CRC remains unexplored. Western blot, quantitative real-time PCR (RT-qPCR) and immunohistochemical (IHC) were used to detect METTL3 expression in cell lines and patient tissues. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) and transcriptomic RNA sequencing (RNA-seq) were used to screen the target genes of METTL3. The biological functions of METTL3 were investigated in vitro and in…

• NN
  National Natural Science Foundation of China

  Awards: U1611261, 81772614, 81871951, 2018YFC1313300
• NS
  Natural Science Foundation of Guangdong Province

  Awards: 2017A030313485, 2014A030312015, 2017A030313485, 2014A030312015, 2018B030306049, 2018A0303130282
• SA
  Science and Technology Planning Project of Guangdong Province

  Award: 2015B020232008