Bulk tissue cell type deconvolution with multi-subject single-cell expression reference

Knowledge of cell type composition in disease relevant tissues is an important step towards the identification of cellular targets of disease. We present MuSiC, a method that utilizes cell-type specific gene expression from single-cell RNA sequencing (RNA-seq) data to characterize cell type compositions from bulk RNA-seq data in complex tissues. By appropriate weighting of genes showing cross-subject and cross-cell consistency, MuSiC enables the transfer of cell type-specific gene expression information from one dataset to another. When applied to pancreatic islet and whole kidney expression data in human, mouse, and rats, MuSiC outperformed existing methods, especially for tissues with closely related cell…

• NI
  National Institutes of Health

  Awards: R01HG006137, R01DK105821, R01DK087635, R01GM125301, R01HL113147, R01DK076077, R01GM108600