articleNature MethodsSep 9, 2019HYBRID OA

High-definition spatial transcriptomics for in situ tissue profiling

SVSanja VickovićGEGökçen EraslanFSFredrik SalménJKJohanna KlughammerLSLinnea Stenbeck

Broad Institute · KTH Royal Institute of Technology · +13 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues. A dense, spatially barcoded bead array captures RNA from histological tissue sections for spatially resolved gene expression analysis.

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Topics & keywords

Topics
Keywords
  • Transcriptome
  • In situ
  • Biology
  • Computational biology
  • High resolution
  • RNA
  • Gene expression profiling
  • Profiling (computer programming)
UN Sustainable Development Goals
  • Good health and well-being
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