HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation
Henan University · Chinese Academy of Sciences · +7 more institutions
Abstract
-cleavage activity against collateral single-stranded DNA (ssDNA) and employed the activity to develop a rapid nucleic acid detection system, namely HOLMES (one-hour low-cost multipurpose highly efficient system). Here, with the employment of thermophilic CRISPR-Cas12b, we create HOLMESv2 for four different applications: (1) specifically discriminating single nucleotide polymorphism (SNP); (2) simply detecting virus RNA, human cell mRNA and circular RNA; (3) conveniently quantitating target nucleic acids with a one-step system combined with LAMP amplification in a constant temperature, thus avoiding cross-contamination; (4) accurately quantitating target DNA methylation degree with the combination of Cas12b…
Citation impact
- FWCI
- 21.62
- Percentile
- 100%
- References
- 20
Authors
7- LLLinxian Li
Henan University, Chinese Academy of Sciences, Shanghai Institutes for Biological Sciences, Center for Excellence in Molecular Plant Sciences, University of Chinese Academy of Sciences
- SLShiyuan Li
- NWNa Wu
Chinese Academy of Sciences, ShanghaiTech University, Shanghai Institutes for Biological Sciences, Center for Excellence in Molecular Plant Sciences, University of Chinese Academy of Sciences
- JWJiacheng Wu
ShanghaiTech University
- GWGang Wang
Henan University
Topics & keywords
- CRISPR
- Nucleic acid
- Computational biology
- Software portability
- DNA methylation
- DNA
- Trans-activating crRNA
- Molecular diagnostics
- Reduced inequalities