reviewGenes & DevelopmentOct 8, 2019DIAMOND OA

Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

California Institute for Regenerative Medicine · University of California, Berkeley · +1 more institution

PubMed
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Abstract

The idea that liquid-liquid phase separation (LLPS) may be a general mechanism by which molecules in the complex cellular milieu may self-organize has generated much excitement and fervor in the cell biology community. While this concept is not new, its rise to preeminence has resulted in renewed interest in the mechanisms that shape and drive diverse cellular self-assembly processes from gene expression to cell division to stress responses. In vitro biochemical data have been instrumental in deriving some of the fundamental principles and molecular grammar by which biological molecules may phase separate, and the molecular basis of these interactions. Definitive evidence is lacking as to whether the same…

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