Single-cell transcriptomics of human T cells reveals tissue and activation signatures in health and disease

Abstract Human T cells coordinate adaptive immunity in diverse anatomic compartments through production of cytokines and effector molecules, but it is unclear how tissue site influences T cell persistence and function. Here, we use single cell RNA-sequencing (scRNA-seq) to define the heterogeneity of human T cells isolated from lungs, lymph nodes, bone marrow and blood, and their functional responses following stimulation. Through analysis of >50,000 resting and activated T cells, we reveal tissue T cell signatures in mucosal and lymphoid sites, and lineage-specific activation states across all sites including distinct effector states for CD8 + T cells and an interferon-response state for CD4 + T cells.…

• AA
  American Association of Immunologists
• NI
  National Institutes of Health

  Awards: AI106697, AI128949, S10RR027050
• NI
  National Institute of Allergy and Infectious Diseases

  Award: AI128949