Long noncoding RNA GAS5 inhibits progression of colorectal cancer by interacting with and triggering YAP phosphorylation and degradation and is negatively regulated by the m6A reader YTHDF3

Abstract Background YAP activation is crucial for cancer development including colorectal cancer (CRC). Nevertheless, it remains unclear whether N6-Methyladenosine (m 6 A) modified transcripts of long noncoding RNAs (lncRNAs) can regulate YAP activation in cancer progression. We investigated the functional link between lncRNAs and the m 6 A modification in YAP signaling and CRC progression. Methods YAP interacting lncRNAs were screened by RIP-sequencing, RNA FISH and immunofluorescence co-staining assays. Interaction between YAP and lncRNA GAS5 was studied by biochemical methods. MeRIP-sequencing combined with lncRNA-sequencing were used to identify the m 6 A modified targets of YTHDF3 in CRC. Gain-of-function…

• NN
  National Natural Science Foundation of China

  Awards: 31570753, 201803040019, U1801282, 81702322, 81802382, 81525020
• CP
  China Postdoctoral Science Foundation

  Award: 2018M643339
• GO
  Government of Jiangsu Province
• NS
  Natural Science Foundation of Jiangsu Province

  Award: BE2016666
• HM
  Health Medical Collaborative Innovation Program of Guangzhou

  Award: 201803040019
• GM
  Guangzhou Municipal Science and Technology Project

  Award: 201803040019