Broad-spectrum resistance to bacterial blight in rice using genome editing
International Rice Research Institute · University of Missouri · +13 more institutions
Abstract
Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genes SWEET11, SWEET13 and SWEET14, the expression of which is required for disease susceptibility. We used CRISPR-Cas9-mediated genome editing to introduce mutations in all three SWEET gene promoters. Editing was further informed by sequence analyses of TALe genes in 63 Xoo strains, which revealed multiple TALe variants for SWEET13 alleles. Mutations were also created in SWEET14, which is also targeted by two…
Citation impact
- FWCI
- 102.86
- Percentile
- 100%
- References
- 46
Authors
22- RORicardo OlivaCorresponding
International Rice Research Institute
- CJChonghui Ji
University of Missouri
- GAGenelou Atienza‐Grande
University of the Philippines Los Baños, International Rice Research Institute
- JCJosé C. Huguet‐Tapia
University of Florida
- ÁLÁlvaro L. Pérez‐Quintero
Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Université de Montpellier, Institut de Recherche pour le Développement, Colorado State University
Topics & keywords
- Xanthomonas oryzae
- Biology
- Genetics
- Gene
- Genome
- Xanthomonas oryzae pv. oryzae
- Genome editing
- CRISPR