Human cohesin compacts DNA by loop extrusion
The University of Texas Southwestern Medical Center · The University of Texas at Austin
Abstract
Cohesin is a chromosome-bound, multisubunit adenosine triphosphatase complex. After loading onto chromosomes, it generates loops to regulate chromosome functions. It has been suggested that cohesin organizes the genome through loop extrusion, but direct evidence is lacking. Here, we used single-molecule imaging to show that the recombinant human cohesin-NIPBL complex compacts both naked and nucleosome-bound DNA by extruding DNA loops. DNA compaction by cohesin requires adenosine triphosphate (ATP) hydrolysis and is force sensitive. This compaction is processive over tens of kilobases at an average rate of 0.5 kilobases per second. Compaction of double-tethered DNA suggests that a cohesin dimer extrudes DNA…
Citation impact
- FWCI
- 35.63
- Percentile
- 100%
- References
- 42
Authors
5- YKYoori KimCorresponding
The University of Texas Southwestern Medical Center
- ZSZhubing ShiCorresponding
The University of Texas Southwestern Medical Center
- HZHongshan Zhang
The University of Texas at Austin
- IJIlya J. FinkelsteinCorresponding
The University of Texas at Austin
- HYHongtao YuCorresponding
The University of Texas Southwestern Medical Center
Topics & keywords
- Cohesin
- Extrusion
- DNA
- Loop (graph theory)
- Cell biology
- Chemistry
- Genetics
- Biology