articleJournal of Extracellular VesiclesNov 29, 2019GOLD OA

Quality and efficiency assessment of six extracellular vesicle isolation methods by nano‐flow cytometry

Xiamen University · Collaborative Innovation Center of Chemistry for Energy Materials

Indexed incrossrefdoaj

Abstract

ABSTRACT Extracellular vesicles (EVs) have sparked tremendous interest owing to their prominent potential in diagnostics and therapeutics. Isolation of EVs from complex biological fluids with high purity is essential to the accurate analysis of EV cargo. Unfortunately, generally used isolation techniques do not offer good separation of EVs from non‐EV contaminants. Hence, it is important to have a standardized method to characterise the properties of EV preparations, including size distribution, particle concentration, purity and phenotype. Employing a laboratory‐built nano‐flow cytometer (nFCM) that enables multiparameter analysis of single EVs as small as 40 nm, here we report a new benchmark to the quality…

No related works found for this paper.

Funding