Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets
Nanchang University · State Key Laboratory of Food Science and Technology · +2 more institutions
Abstract
Beyond its extraordinary genome editing ability, the CRISPR-Cas systems have opened a new era of biosensing applications due to its high base resolution and isothermal signal amplification. However, the reported CRISPR-Cas sensors are largely only used for the detection of nucleic acids with limited application for non-nucleic-acid targets. To realize the full potential of the CRISPR-Cas sensors and broaden their applications for detection and quantitation of non-nucleic-acid targets, we herein report CRISPR-Cas12a sensors that are regulated by functional DNA (fDNA) molecules such as aptamers and DNAzymes that are selective for small organic molecule and metal ion detection. The sensors are based on the…
Citation impact
- FWCI
- 22.47
- Percentile
- 100%
- References
- 46
Authors
9- YXYing Xiong
Nanchang University, State Key Laboratory of Food Science and Technology
- YXYing XiongCorresponding
Nanchang University, State Key Laboratory of Food Science and Technology, Nanjing University
- JZJingjing ZhangCorresponding
University of Illinois Urbana-Champaign, Nanjing University
- ZYZhenglin Yang
University of Illinois Urbana-Champaign
- QMQuanbing Mou
University of Illinois Urbana-Champaign
Topics & keywords
- CRISPR
- Chemistry
- Nucleic acid
- Trans-activating crRNA
- Molecular beacon
- Deoxyribozyme
- Aptamer
- DNA