Semi-quantitative Determination of Protein Expression Using Immunohistochemistry Staining and Analysis: An Integrated Protocol
University of Oklahoma Health Sciences Center
Abstract
Semi-quantitative IHC is a powerful method for investigating protein expression and localization within tissues. The semi-quantitative immunohistochemistry (IHC) involves using software such as free software ImageJ Fiji to conduct deconvolution and downstream analysis. Currently, there is lack of an integrated protocol that includes a detailed procedure of how to measure or compare protein expression. Publications that use semi-quantification methods to quantify protein expression often don't provide enough details in their methods section, which makes it difficult for the reader to reproduce their data. The current protocol for the first time provides a detailed, step-by-step instruction of conducting…
Citation impact
- FWCI
- 21.32
- Percentile
- 100%
- References
- 17
Authors
2Topics & keywords
- Protocol (science)
- Computer science
- Software
- Immunohistochemistry
- Quantitative analysis (chemistry)
- Protein expression
- Expression (computer science)
- Data mining