Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

Giobbe, Giovanni Giuseppe; Crowley, Claire; Luni, Camilla; Campinoti, Sara; Khedr, Moustafa; Kretzschmar, Kai; Santis, Martina M. De; Zambaiti, Elisa; Michielin, Federica; Meran, Laween; Hu, Qianjiang; Son, Gijs J. F. van; Urbani, Luca; Anna, Manfredi; Giomo, Monica; Eaton, Simon; Cacchiarelli, Davide; Li, Vivian; Clevers, Hans; Bonfanti, Paola; Elvassore, Nicola; Coppi, Paolo De

doi:10.1038/s41467-019-13605-4

articleNature CommunicationsDec 11, 2019GOLD OA

Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

GGGiovanni Giuseppe Giobbe CCClaire Crowley CLCamilla Luni SCSara Campinoti MKMoustafa Khedr

University College London · ShanghaiTech University · +11 more institutions

PubMed

Indexed incrossrefdatacitedoajpubmed

Abstract

Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic,…

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Topics

Keywords

Organoid
Decellularization
Extracellular matrix
Regenerative medicine
Cell biology
Self-healing hydrogels
Tissue engineering
Chemistry

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