U1 snRNP regulates cancer cell migration and invasion in vitro
Howard Hughes Medical Institute · University of Pennsylvania
Abstract
Stimulated cells and cancer cells have widespread shortening of mRNA 3'-untranslated regions (3'UTRs) and switches to shorter mRNA isoforms due to usage of more proximal polyadenylation signals (PASs) in introns and last exons. U1 snRNP (U1), vertebrates' most abundant non-coding (spliceosomal) small nuclear RNA, silences proximal PASs and its inhibition with antisense morpholino oligonucleotides (U1 AMO) triggers widespread premature transcription termination and mRNA shortening. Here we show that low U1 AMO doses increase cancer cells' migration and invasion in vitro by up to 500%, whereas U1 over-expression has the opposite effect. In addition to 3'UTR length, numerous transcriptome changes that could…
Citation impact
- FWCI
- 478.28
- Percentile
- 100%
- References
- 56
Authors
11- JOJung‐Min OhCorresponding
Howard Hughes Medical Institute, University of Pennsylvania
- CCChristopher C. Venters
Howard Hughes Medical Institute, University of Pennsylvania
- CDChao Di
Howard Hughes Medical Institute, University of Pennsylvania
- AMAnna Maria Pinto
Howard Hughes Medical Institute, University of Pennsylvania
- LWLili Wan
Howard Hughes Medical Institute, University of Pennsylvania
Topics & keywords
- Polyadenylation
- Biology
- RNA splicing
- Exon
- Untranslated region
- Transcription (linguistics)
- snRNP
- Cell biology