Eight high-quality genomes reveal pan-genome architecture and ecotype differentiation of Brassica napus

Rapeseed (Brassica napus) is the second most important oilseed crop in the world but the genetic diversity underlying its massive phenotypic variations remains largely unexplored. Here, we report the sequencing, de novo assembly and annotation of eight B. napus accessions. Using pan-genome comparative analysis, millions of small variations and 77.2-149.6 megabase presence and absence variations (PAVs) were identified. More than 9.4% of the genes contained large-effect mutations or structural variations. PAV-based genome-wide association study (PAV-GWAS) directly identified causal structural variations for silique length, seed weight and flowering time in a nested association mapping population with ZS11…

• NN
  National Natural Science Foundation of China

  Awards: 2019CFA014, 31871269, 31571704
• NS
  Natural Science Foundation of Hubei Province

  Awards: 2019CFA014, 31871269
• HA
  Huazhong Agricultural University

  Award: 2019CFA014
• NK
  National Key Research and Development Program of China

  Awards: 2016YFD0101000, 2015CB150200
• FR
  Fundamental Research Funds for the Central Universities

  Awards: 2662018PY068, 2662017PY043